Electron Microscopic In Situ DNA Nick End-labeling in Combination with Immunoelectron Microscopy
نویسندگان
چکیده
منابع مشابه
"Apoptotic" myocytes in infarct area in rabbit hearts may be oncotic myocytes with DNA fragmentation: analysis by immunogold electron microscopy combined with In situ nick end-labeling.
BACKGROUND Modes of cell death have been defined morphologically as apoptosis and oncosis. Infarcted myocytes have been reported to show apoptosis, as revealed by DNA fragmentation by DNA ladder and by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) at the light microscopic level. We investigated whether TUNEL-positive infarcted myocytes have apoptotic or o...
متن کاملSignificance of myocytes with positive DNA in situ nick end-labeling (TUNEL) in hearts with dilated cardiomyopathy.
Situ Nick End-Labeling (TUNEL) in Hearts With Dilated Cardiomyopathy To the Editor: Recently, Kanoh et al1 seriously questioned the reliability of the DNA in situ nick end-labeling (TUNEL) assay as a method of detecting cardiomyocyte apoptosis (CA) in dilated cardiomyopathy. Our experience with both failing2 and infarcted3 human hearts is remarkably different. We agree that TUNEL positivity is ...
متن کاملGlomerular changes in microscopic haematuria, studied by quantitative immunoelectron microscopy and in situ zymography.
BACKGROUND Haematuria of glomerular origin, even if mild, implies the development of defects in the glomerular basement membrane (GBM). In diseases where there is no infiltration of leukocytes into the glomerulus-such as thin basement membrane disease (TBMD) and histologically mild cases of IgA nephropathy (IgAN)-the mechanism by which such defects form is unclear. METHODS Frozen renal tissue...
متن کاملSignificance of myocytes with positive DNA in situ nick end-labeling (TUNEL) in hearts with dilated cardiomyopathy: not apoptosis but DNA repair.
BACKGROUND The presence of apoptotic myocytes has been reported in human hearts with dilated cardiomyopathy (DCM) on the basis of a positive finding of DNA in situ nick end-labeling (TUNEL). However, ultrastructural evidence of myocyte apoptosis has not been obtained. METHODS AND RESULTS A total of 80 endomyocardial biopsies were obtained from right and left ventricles of 20 patients with DCM...
متن کاملDetection of DNA damage in prokaryotes by terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling.
Numerous agents can damage the DNA of prokaryotes in the environment (e.g., reactive oxygen species, irradiation, and secondary metabolites such as antibiotics, enzymes, starvation, etc.). The large number of potential DNA-damaging agents, as well as their diverse modes of action, precludes a simple test of DNA damage based on detection of nucleic acid breakdown products. In this study, free 3'...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1999
ISSN: 0022-1554,1551-5044
DOI: 10.1177/002215549904700513